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Characterization of porphyrins of rat Harderian gland by thin layer chromatography and mass spectrometry: no evidence for a tricarboxylic acid porphyrin
Author(s) -
Gorchein Abel
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.276
Subject(s) - chemistry , porphyrin , protoporphyrin , derivatization , chromatography , protoporphyrin ix , mass spectrometry , electrospray ionization , hemeprotein , heme , thin layer chromatography , reagent , biochemistry , organic chemistry , enzyme , photodynamic therapy
Reversed‐phase TLC systems using ion‐pairing reagents are described for the separation without prior derivatization of the porphyrins of rat Harderian gland. Porphyrins with R f values greater than protoporphyrin but smaller than coproporphyrin, representing harderoporphyrin, were labile to 1 m NaOH, which converted them to protoporphyrin, inconsistent with the established literature that harderoporphyrin is a tricarboxylic acid porphyrin. It was conrmed by HPLC/electrospray ionization MS that this ‘harderoporphyrin’ fraction consists of the recently characterized glycoconjugate, protoporphyrin‐1‐ O ‐acyl β ‐xyloside, with trace amounts of protoporphyrin‐1‐ O ‐acyl β ‐glucoside. Copyright © 2003 John Wiley & Sons, Ltd.