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Ultra‐performance liquid chromatography‐tandem mass spectrometry method for the determination of febuxostat in dog plasma and its application to a pharmacokinetic study
Author(s) -
Zhang Tianhong,
Sun Yuanpeng,
Zhang Peng,
Gao Jingmei,
Wang Shanshan,
He Zhonggui
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2756
Subject(s) - chromatography , chemistry , febuxostat , formic acid , high performance liquid chromatography , pharmacokinetics , extraction (chemistry) , liquid chromatography–mass spectrometry , mass spectrometry , selected reaction monitoring , tandem mass spectrometry , beagle , ethyl acetate , pharmacology , uric acid , medicine , biochemistry , hyperuricemia
A rapid, sensitive and selective ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for the determination of febuxostat in dog plasma. Using paclitaxel as an internal standard (IS), a simple liquid–liquid extraction method with ethyl acetate was adopted for plasma sample pretreatment. Separation was carried out on an Acquity UPLC BEH C 18 column with a mobile phase consisting of acetonitrile and water (containing 0.2% formic acid). The assay was linear in the concentration ranged from 5 to 5000 ng/mL with a lower limit of quantification of 5 ng/mL for febuxostat. The single run analysis was as short as 2.0 min. Finally, the developed method was successfully applied to the pharmacokinetic study of febuxostat tablets following oral administration at a single dose of 40 mg in beagle dogs. Copyright © 2012 John Wiley & Sons, Ltd.