Premium
In vivo metabolism study of vaccarin in rat using HPLC‐LTQ‐MS n
Author(s) -
Qi Peng,
Fan Mingsong,
Li Zhixiong,
Chen Mingcang,
Sun Zhaolin,
Wu Bin,
Huang Chenggang
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2753
Subject(s) - chemistry , hydroxylation , in vivo , chromatography , biotransformation , metabolism , metabolite , electrospray ionization , high performance liquid chromatography , urine , mass spectrometry , metabolic pathway , drug metabolism , glycosylation , biochemistry , enzyme , microbiology and biotechnology , biology
In order to illustrate the main biotransformation pathways of vaccarin in vivo , metabolites of vaccarin in rats were identified using a specific and sensitive high‐performance liquid chromatography–electrospray ionization linear ion trap mass spectrometry (LTQ XL™) method. The rats were administered a single dose (200 mg/kg) of vaccarin by oral gavage. By comparing their changes in molecular masses (Δ M ), retention times and spectral patterns with those of the parent drug, the parent compound and six metabolites were found in rat urine after oral administration of vaccarin. The parent compound and five metabolites were detected in rat plasma. In heart, liver and kidney samples, respectively, one, four and three metabolites were identified, in addition to the parent compound. Three metabolites, but no trace of parent drug, were found in the rat feces. This is the first systematic metabolism study of vaccarin in vivo . The biotransformation pathways of vaccarin involved methylation, hydroxylation, glycosylation and deglycosylation. Copyright © 2012 John Wiley & Sons, Ltd.