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Development of rapid and simultaneous quantitative method for green tea catechins on the bioanalytical study using UPLC/ESI‐MS
Author(s) -
Misaka Shingen,
Kawabe Keisuke,
Onoue Satomi,
Werba José Pablo,
Giroli Monica,
Kimura Junko,
Watanabe Hiroshi,
Yamada Shizuo
Publication year - 2013
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2740
Subject(s) - chemistry , chromatography , bioanalysis , electrospray ionization , pharmacokinetics , catechin , epigallocatechin gallate , calibration curve , green tea , epicatechin gallate , quantitative analysis (chemistry) , detection limit , mass spectrometry , polyphenol , antioxidant , pharmacology , food science , biochemistry , medicine
A rapid and quantitative analytical method for the simultaneous determination of green tea catechins using ultra‐performance liquid chromatography/electrospray ionization–mass spectrometry was developed. Total analytical run time was 3.5 min for the detection of (−)‐epicatechin (EC), (−)‐epicatechin‐3‐ O ‐gallate (ECG), (−)‐epigallocatechin (EGC), (−)‐epigallocatechin‐3‐ O ‐gallate (EGCG) and myricetin as the internal standard (IS) in rat plasma. The calibration curves were linear over the range of 10–5000 ng/mL for all the catechins. The inter‐ and intra‐day precision (relative standard deviation) and accuracy (percentage deviation) of the method were both lower than 10%. The average extraction recoveries in plasma ranged from 68.5 to 86.5%, and the lower limits of quantification of EC, EGC, ECG and EGCG were 10 ng/mL with a signal‐to‐noise ratio of >10. The assay developed was successfully applied to a pharmacokinetic study of catechins following intravenous and intragastric administrations of green tea extract in rats. Plasma concentrations of four catechins were detected up to 5–24 h after administration, and the pharmacokinetic parameters of catechins were in agreement with previous studies. From these findings, taken together with the high productivity and precision, the developed method could be a reliable and reproducible tool for the evaluation of pharmacokinetic properties of catechins. Copyright © 2012 John Wiley & Sons, Ltd.