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Simultaneous determination of lactate and pyruvate in human sweat using reversed‐phase high‐performance liquid chromatography: a noninvasive approach
Author(s) -
Biagi Simona,
Ghimenti Silvia,
Onor Massimo,
Bramanti Emilia
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2713
Subject(s) - chemistry , chromatography , high performance liquid chromatography , sweat , calibration curve , detection limit , quantitative analysis (chemistry) , medicine
The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high‐performance liquid chromatography (RP‐HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 ‐ 350 m m of lactate, 0.003‐ 1 m m of pyruvate. The sensitivity was good with a limit of detection of 0.03 m m for lactate and 0.001 m m for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. Copyright © 2012 John Wiley & Sons, Ltd.