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Liquid chromatography/tandem mass spectrometry method for quantification of trans ‐stilbene glycoside in rat plasma and its pharmacokinetic application
Author(s) -
Wang Jiankun,
Zhu Xuanxuan,
Peng Ying,
Zha Weibin,
Feng Dong,
Zhu Yinci,
Wan Ping,
Qi Huanhuan,
He Jichao,
Zhou Jie,
Sun Jianguo
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2707
Subject(s) - chemistry , chromatography , protein precipitation , selected reaction monitoring , pharmacokinetics , tandem mass spectrometry , analyte , mass spectrometry , extraction (chemistry) , detection limit , elution , liquid chromatography–mass spectrometry , high performance liquid chromatography , coefficient of variation , medicine
ABSTRACT A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the quantification of trans ‐stilbene glycoside (SG) in rat plasma. As trans ‐SG can be rapidly isomerized under light exposure, trans ‐SG plasma samples were prepared in the dark and assayed immediately. Trans ‐SG and internal standard were extracted by protein precipitation. Chromatographic separation was achieved on a C 18 column with a gradient elution program. The detection of analytes was performed by negative ion via multiple reaction monitoring mode. The precursor‐to‐product ions of m/z 405.1 → 242.9 for trans ‐SG and m/z 389.1 → 226.9 for polydatin (internal standard) were monitored. No interference of endogenous components was observed for any plasma samples that we studied.The method was linear over the concentration range of 1.0–1000.0 ng/mL with a good correlation coefficient. The lower limit of quantification was 1.0 ng/mL for trans ‐SG. The intra and inter‐batch accuracy for trans ‐SG in stable rat plasma samples ranged from 93.3 to 102.7% and the variation was less than 8.1%. The extraction recoveries of trans ‐SG in rat plasma were from 102.8 to 112.4% and the matrix effects were also acceptable. This method was successfully applied to pharmacokinetic study of trans ‐SG in rats after intravenous administration. Copyright © 2012 John Wiley & Sons, Ltd.