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Validated LC‐MS/MS assay for the quantitative determination of clematichinenoside AR in rat plasma and its application to a pharmacokinetic study
Author(s) -
Wang Dawei,
Li Feng,
Li Pei,
Zhang Ji,
Liu Li,
Xu Ping,
Zhou Lei,
Liu Xiaodong
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.2691
Subject(s) - chemistry , chromatography , electrospray ionization , pharmacokinetics , extraction (chemistry) , selected reaction monitoring , acetic acid , mass spectrometry , acetonitrile , tandem mass spectrometry , liquid chromatography–mass spectrometry , elution , analytical chemistry (journal) , medicine , biochemistry
This study aimed to develop and validate a liquid chromatography tandem mass spectrometry method for measuring clematichinenoside AR in rat plasma. Clematichinenoside AR was extracted by solid‐phase extraction and chromatographed on an XTerra MS C 8 column. Pulchinenoside B4 was used as the internal standard. Elution was achieved using an isocratic mobile phase of acetonitrile with 0.1% acetic acid (21:79, v/v) at a flow‐rate of 0.2 mL/min. The detection was performed by multiple reaction monitoring mode via a negative electrospray ionization interface. Standard curves were linear, ranging from 2.5 to 500 ng/mL. The intra‐ and inter‐day precision values were <14.0% and the accuracy was within ±13%. Extraction recovery ranged from 93.2 to 93.9%. This proposed method was successfully applied to a pharmacokinetic study on clematichinenoside AR in rats after oral administration. Copyright © 2012 John Wiley & Sons, Ltd.