Validation of a microscale extraction and high‐throughput UHPLC‐QTOF‐MS analysis method for huperzine A in  Huperzia | Zendy

Cuthbertson Daniel | Zendy; PiljacŽegarac Jasenka | Zendy; Lange Bernd Markus | Zendy

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Validation of a microscale extraction and high‐throughput UHPLC‐QTOF‐MS analysis method for huperzine A in Huperzia

Author(s) -

Cuthbertson Daniel,

PiljacŽegarac Jasenka,

Lange Bernd Markus

Publication year - 2012

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.2677

Subject(s) - huperzine a , chromatography , microscale chemistry , extraction (chemistry) , detection limit , chemistry , throughput , linear range , acetylcholinesterase , computer science , biochemistry , mathematics , enzyme , mathematics education , telecommunications , wireless

Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase‐inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC‐QTOF‐MS method for the high‐throughput analysis of H . squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20–10,000 pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources. Copyright © 2012 John Wiley & Sons, Ltd.

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