Premium
Determination of the compositions of polysaccharides from Chinese herbs by capillary zone electrophoresis with amperometric detection
Author(s) -
Wang Qingjiang,
Ding Fei,
Zhu Ningning,
He Pingang,
Fang Yuzhi
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.267
Subject(s) - chemistry , monosaccharide , capillary electrophoresis , rhamnose , polysaccharide , arabinose , chromatography , xylose , galactose , amperometry , fucose , hydrolysis , fermentation , biochemistry , electrode , electrochemistry
Abstract In this paper, capillary zone electrophoresis with amperometric detection (CZE‐AD) was applied to determine the compositions of hetero‐polysaccharides from Chinese herbs, Angelica sinensis and ax by analyzing their hydrolyzed monosaccharides: fucose, galactose, glucose, arabinose, rhamnose and xylose. Under the selected optimum conditions, the six monosaccharides could be perfectly separated within 25 min and showed signicant current responses at copper electrodes. The linear ranges of the six monosaccharides were all from 5.0 × 10 −6 to 2.0 × 10 −4 mol L −1 and their detection limits were lower or near 1.0 × 10 −6 mol L −1 (S/N = 3). Experiments showed that the Angelica sinensis polysaccharide was composed of fucose, galactose, glucose, arabinose, rhamnose and xylose (mole ratio 1.0:13.6:15.0:8.7:21.3:3.7), and the ax polysaccharide was composed of galactose, glucose and arabinose (mole ratio 1.0:4.98:1.1). The purity of these polysaccharides leached by the introduced leaching method was 98.3 and 97.6%, respectively. Analyzing polysaccharides by this method has some merits of speed, simple instrumentation and operation, high sensitivity and high reproducibility. Copyright © 2003 John Wiley & Sons, Ltd.