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Improvement of cyclosporin A determination in whole blood by reversed‐phase high‐performance liquid chromatography
Author(s) -
Ouyang Jin,
Baeyens Willy R. G.,
Duan Jingli,
Delanghe Joris
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.262
Subject(s) - chromatography , chemistry , pharmacokinetics , detection limit , high performance liquid chromatography , calibration curve , relative standard deviation , whole blood , quantitative analysis (chemistry) , pharmacology , surgery , medicine
A chromatographic method was developed for the determination of cyclosporin A in human whole blood using reversed‐phase HPLC at room temperature. Most previous reports carried out this liquid chromatographic separation at temperatures above 70°C. The present procedure greatly improves the detection limit by controlling peak broadening effects, as well as the lifetime of the column at room temperature. Under optimal conditions and using ketoconazole as an internal standard, the calibration graph was linear in the range of 16–1000 µg/L with a relative standard deviation of 3.72% at 150 µg/L and 2.45% at 300 µg/L ( n = 11) of cyclosporin A. The detection limit was of 5.0 µg/L cyclosporin A. By this procedure, cyclosporin A pharmacokinetic parameters in healthy Chinese subjects were studied. The developed method could be applied to the quantication of cyclosporin A in human blood samples and allows the study of its pharmacokinetics in routine laboratories. Copyright © 2003 John Wiley & Sons, Ltd.