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Reversed‐phase liquid chromatographic method for the analysis of aminoglycoside antibiotics using pre‐column derivatization with phenylisocyanate
Author(s) -
Kim ByoungHyoun,
Lee Suk Chin,
Lee Hye Jin,
Ok Jong Hoa
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.254
Subject(s) - phenylisocyanate , chromatography , derivatization , chemistry , aminoglycoside , high performance liquid chromatography , mass spectrometry , antibiotics , organic chemistry , biochemistry
A pre‐column derivatization liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics using phenylisocyanate as a derivatization reagent. Derivatives including kanamycin, neomycin and gentamicin were formed by reaction of the analytes with phenylisocyanate in the presence of triethylamine. Phenylisocyanato groups were attached to corresponding amino groups of aminoglycoside and their molecular mass was conrmed by liquid chromatography–electrospray ionization–mass spectrometry (LC‐ESI‐MS). The experimental conditions for derivatization and separation of aminoglycoside derivatives were optimized and validated. A simple liquid chromatographic method for the determination of aminoglycoside antibiotics was demonstrated. Copyright © 2003 John Wiley & Sons, Ltd.