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Molecular characterization of two host–guest associating hyaluronan derivatives
Author(s) -
Šoltés L.,
Mendichi R.
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.252
Subject(s) - chemistry , radius of gyration , size exclusion chromatography , hydrodynamic radius , chromatography , cyclodextrin , derivative (finance) , molar mass distribution , polymer chemistry , nuclear chemistry , organic chemistry , polymer , economics , financial economics , copolymer , enzyme
Molecular characteristics were determined of two high‐molecular‐weight water‐soluble hyaluronan derivatives, namely β ‐cyclodextrin (HA‐ β ‐CD) and N‐acylurea (EDC‐HA). The weight‐average molecular weight ( M w ) of HA‐ β ‐CD and of EDC‐HA, determined with a multi‐angle light scattering detector connected on‐line to a size exclusion chromatographic system, was respectively 185.3 and 86.8 kDa. However the M w value determined for the equimolar mixture of the two HA derivatives equaled 556.0 kDa. Similarly, the gyration radius of the above equimolar mixture, Rg = 80.6 nm, was signicantly greater than the values found for the single HA derivative, i.e. 40.2 nm for HA‐ β ‐CD and 23.8 nm for EDC‐HA. These data indicate that the two kinds of substituents, bound to the polymeric chains, form host–guest/inclusion complexes resulting in polymacromolecular associates/aggregates. Copyright © 2003 John Wiley & Sons, Ltd.