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Analysis of glycopeptide antibiotics using micellar electrokinetic chromatography and borate complexation
Author(s) -
Lucas Carmelle,
Foley Joe P.,
Ahuja Eric S.
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.235
Subject(s) - chemistry , chromatography , micellar electrokinetic chromatography , glycopeptide , sodium dodecyl sulfate , ristocetin , micelle , boron , vancomycin , detection limit , antibiotics , aqueous solution , organic chemistry , biochemistry , staphylococcus aureus , bacteria , platelet , genetics , platelet aggregation , immunology , biology
Abstract Micellar electrokinetic chromatography (MEKC) was investigated as a technique for the separation and analysis of the following related glycopeptide antibiotics: α‐avoparcin, β‐avoparcin, ristocetin A, ristocetin B and vancomycin. Sodium dodecyl sulfate (SDS) micelles were employed as the pseudostationary phase in conjunction with borate or CHES buffers at pH 9.2. A complete separation of the glycopeptides was achieved only when two separation mechanisms were employed simultaneously: (i) differential partitioning of the glycopeptides into SDS micelles; and (ii) differential complexation of the glycopeptides with the borate anion from the borate buffer. Quantitatively, linearity was confirmed for each antibiotic from 0.5 to 40 ppm, with correlation coefficients ( r 2 ) ranging from 0.9996 (vancomycin and β‐avoparcin) to 0.9986 (α‐avoparcin). Detection limits ranging from 0.01 ppm (vancomycin) to 0.2 ppm (avoparcin) were achieved, and the mean recovery of avoparcin at the 10 ppm level was 99.2%. Copyright © 2003 John Wiley & Sons, Ltd.