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Lophine derivatives as versatile analytical tools
Author(s) -
Nakashima Kenichiro
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.226
Subject(s) - chemistry , reagent , chloride , metal ions in aqueous solution , benzoyl chloride , boronic acid , phenols , combinatorial chemistry , chemiluminescence , organic chemistry , chromatography , metal
Lophine (2,4,5‐triphenylimidazole) derivatives as versatile analytical tools in biomedical sciences are described. Chemiluminescence (CL) and fluorescence (FL) properties of the lophine derivatives are first demonstrated including the CL reaction mechanism, effects of substituents on CL yields, FL spectral behaviors, etc. Next, analytical applications to the determination of metal ions such as cobalt (II) and chromium (VI) are discussed. Finally, the application studies of lophine derivatives as CL and FL reagents for the determination of organic substances in biological materials are presented. Among the derivatives, 2‐(4‐hydrazinocarbonylphenyl)‐4,5‐diphenylimidazole (HCPI) and 4‐(4,5‐diphenyl‐1 H ‐imidazol‐2‐yl)benzoyl chloride (DIB‐Cl) are studied, with their excellent properties as labeling reagents for fatty acids and amines and/or phenols, respectively, in high‐performance liquid chromatography. The utility of boronic acid derivatives as CL enhancers is also discussed in this review. Copyright © 2003 John Wiley & Sons, Ltd.