Premium
Preliminary study on application of impregnated synthetic peptide TLC stationary phases for the pre‐screening of 5‐HT 1A ligands
Author(s) -
Zajdel PaweŁ,
Bojarski Andrzej J.,
Byrtus Hanna,
ChŁońRzepa Grażyna,
Obniska Jolanta,
Chevallet Pierre,
Martinez Jean,
PawŁowski Maciej
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.224
Subject(s) - chemistry , tetrapeptide , chromatography , peptide , residue (chemistry) , silica gel , amino acid , amino acid residue , peptide sequence , organic chemistry , biochemistry , gene
Chromatographic parameters (Δ R f ), defined as a difference in the migration of tested compound on the control and peptide impregnated silica gel TLC plates, were determined for 42 arylpiperazine derivatives. An amino acid sequence of the peptide used for impregnation was derived from the III transmembrane segment of the 5‐HT 1A receptor in the close vicinity of aspartic acid (Asp 166) residue. It was found that the Δ R f values obtained in a model employing tetrapeptide P4LA (ADVL), as well as the calculated log P correlate with 5‐HT 1A receptor affinity of the studied compounds. Copyright © 2003 John Wiley & Sons, Ltd.