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Quantitation of oxcarbazepine and its metabolites in human plasma by micellar electrokinetic chromatography
Author(s) -
Pucci Vincenzo,
Kenndler Ernst,
Raggi Maria Augusta
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.217
Subject(s) - chromatography , chemistry , oxcarbazepine , detection limit , repeatability , analyte , micellar electrokinetic chromatography , extraction (chemistry) , solid phase extraction , carbamazepine , neuroscience , epilepsy , biology
A reliable micellar electrokinetic chromatographic method for the determination of oxcarbazepine and its two main metabolites, 10‐hydroxycarbamazepine and 10,11‐ trans ‐dihydroxy‐10,11‐dihydroxycarbamazepine, in human plasma was developed. The separation and determination of the analytes was achieved using a system consisting of 60 m M SDS in phosphate buffer (30 m M , pH 8.0), to which 20% (v/v) methanol was added. Separation was carried out in an uncoated fused‐silica capillary with a separation voltage of 25 kV and currents typically less than 40 µA. Spectrophotometric detection was at 205 nm. Isolation of oxcarbazepine and its metabolites from plasma was accomplished by a solid‐phase extraction procedure. The mean extraction yield of the analytes from plasma was higher than 94%. The linear correlation coefficients were better than 0.994 for all analytes. The limit of detection was 0.05 µg/mL, the limit of quantitation 0.15 µg/mL. The repeatability for the spiked blank plasma samples was lower than 1.9% and the intermediate precision lower than 2.1%, both expressed as RSD%. The results obtained analysing real plasma samples from epileptic patients under therapy with Tolep® were satisfactory in terms of precision, accuracy and detectability. Copyright© 2003 John Wiley & Sons, Ltd.