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Laser‐induced fluorescence as a powerful detection tool for capillary electrophoretic analysis of heparin/heparan sulfate disaccharides
Author(s) -
Militsopoulou Maria,
Lecomte Céline,
Bayle Christopher,
Couderc Francois,
Karamanos Nikos K.
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.207
Subject(s) - chemistry , capillary electrophoresis , heparan sulfate , detection limit , chromatography , fluorophore , heparin , laser induced fluorescence , fluorescence , disaccharide , electrophoresis , quantitative analysis (chemistry) , biochemistry , physics , quantum mechanics
Abstract In quest for high sensitivities necessary for determining the disaccharide composition of heparin/heparan sulfate present in trace amounts in biologic samples, an ultrahighly sensitive capillary electrophoresis (CE) method using laser‐induced fluorescence (LIF) detection was developed. Heparin/heparan sulfate‐derived Δ‐disaccharides were derivatized with the fluorophore 2‐aminoacridone and resolved by a reversed‐polarity CE method. Estimation of the limit of detection in concentration term and limit of quantitation showed that LIF detection of AMAC‐derivatives of Δ‐disaccharides resulted in 27–744 times higher sensitivity as compared to those detected by UV at 255 nm. These data suggest that CE‐LIF is a powerful tool to quantify minute amounts of heparin/heparan sulfate disaccharides. Copyright © 2003 John Wiley & Sons, Ltd.