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Synthesis and analysis of aminochromes by HPLC‐photodiode array. Adrenochrome evaluation in rat blood
Author(s) -
Remião Fernando,
Milhazes Nuno,
Borges Fernanda,
Carvalho Félix,
Bastos Maria L.,
LemosAmado Francisco,
Domingues Pedro,
FerrerCorreia A.
Publication year - 2003
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.203
Subject(s) - adrenochrome , chemistry , high performance liquid chromatography , chromatography , autoxidation , catecholamine , amperometry , biochemistry , electrochemistry , epinephrine , endocrinology , electrode , medicine
The catecholamine oxidation process induces cardiotoxicity and neurotoxicity. Catecholamines can oxidize to aminochromes through autoxidation or by enzymatic or non‐enzymatic catalysis. Although some toxic effects seem to be related to the formation of aminochromes there is still scarce information concerning the identification and evaluation of these compounds in in vivo models. In this study five catecholamines were oxidized to their respective aminochromes: adrenaline/adrenochrome; noradrenaline/noradrenochrome; dopa/dopachrome; dopamine/dopaminochrome; and isoproterenol/isoprenochrome. The evaluation of the catecholamines oxidation profile was performed by HPLC with photodiode array detection and using either enzymatic (tyrosinase) or non‐enzymatic [Ag 2 O, CuSO 4 , NaIO 4 and K 3 Fe(CN) 6 ] catalytic systems. The NaIO 4 was found to be the most efficient oxidant of catecholamines. An isocratic reverse‐phase HPLC method was developed to analyse each pair of catecholamine–aminochrome. The analytical system was then applied to the detection of adrenochrome in rat blood at 490 nm. Thus, adrenochrome was administered i.p. to rats and its concentration in whole blood was monitored after 5, 15 and 25 min. Blood treatment for adrenochrome evaluation consists of an acidification for protein precipitation followed by a rapid neutralization. The results showed a rapid decrease of adrenochrome concentration in blood after its administration. The adrenochrome present in blood was characterized by UV and tandem mass spectrometry. Copyright © 2002 John Wiley & Sons, Ltd.