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Screening for 18 diuretics and probenecid in doping analysis by liquid chromatography–tandem mass spectrometry
Author(s) -
Deventer K.,
Delbeke F. T.,
Roels K.,
Van Eenoo P.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.201
Subject(s) - chemistry , triamterene , chromatography , bumetanide , hydrochlorothiazide , probenecid , furosemide , liquid chromatography–mass spectrometry , mass spectrometry , tandem mass spectrometry , amiloride , pharmacology , sodium , endocrinology , medicine , cotransporter , organic chemistry , blood pressure
A fast and selective liquid chromatography–tandem mass spectrometric (LC/MS/MS) method for the screening of 18 diuretics and probenecid in human urine is presented. Analyses were performed on a LCQ‐Deca® instrument equipped with ESI‐interface using scan by scan polarity changing. All diuretics and probenecid were separated in less than 20 min after liquid–liquid extraction with ethyl acetate. The LOD for all substances was 100 ng/mL or better. The method was applied to detect diuretics after the oral administration of several drugs including hydrochlorothiazide, bumetanide, spironolactone, furosemide, amiloride, triamterene, chlortalidone and epithizide. All diuretics could be detected for periods up to 96 h after the intake of therapeutic amounts. Copyright © 2002 John Wiley & Sons, Ltd.