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Molecular characteristics of some commercial high‐molecular‐weight hyaluronans
Author(s) -
Šoltés L.,
Mendichi R.,
Lath D.,
Mach M.,
Bakoš D.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.185
Subject(s) - chemistry , chromatography , size exclusion chromatography , photometer , viscometer , elution , gradient elution , molecular mass , gel permeation chromatography , capillary action , light scattering , analytical chemistry (journal) , high performance liquid chromatography , scattering , polymer , organic chemistry , optics , viscosity , physics , materials science , quantum mechanics , composite material , enzyme
Commercially available hyaluronan (HA) samples were investigated by the method of size exclusion chromatography (SEC). The fractions eluted from the SEC column were on‐line molecularly characterized by using a multi‐angle laser light scattering (MALLS) photometer. Along with the SEC‐MALLS technique, the high‐molecular‐weight HA biopolymers were (off‐line) analyzed by capillary viscometry. Copyright © 2002 John Wiley & Sons, Ltd.