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Analysis of glucose and lactate in dialysate from hypothalamus of rats after exhausting swimming using microdialysis
Author(s) -
Wang L.,
Dong Y.,
Yu X.,
Shangguan D. H.,
Zhao R.,
Han H. W.,
Liu G. Q.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.177
Subject(s) - microdialysis , chemistry , basal (medicine) , chromatography , hypothalamus , extracellular fluid , medicine , endocrinology , extracellular , biochemistry , insulin
A microbore flow injection analysis–immobilized enzyme reactor–electrochemical detection (FIA‐IMER‐ECD) system for glucose and lactate detection was built up. The assays were precise, sensitive and practicable for determination of glucose and lactate levels in hypothalamic dialysate. The method had been used to detect the dynamic changes of glucose and lactate levels during rat exhausting swimming and recovery. The data showed that after exhausting swimming, the concentration of glucose in hypothalamic dialysate that reflected the concentration in the hypothalamic extracellular fluid decreased. The level fell to its nadir at day 1 after the exercise and then went back to the basal level at day 3 after the swimming. However, lactate levels increased to a maximum at day 3 and went back to the basal level at day 5 after the swimming. Copyright © 2002 John Wiley & Sons, Ltd.