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Semi‐micro liquid chromatography of aromatic amino acid metabolites using isocratic elution and Column switching
Author(s) -
Hanai Toshihiko,
Kaneko Kazuko,
Homma H.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.176
Subject(s) - chemistry , chromatography , catecholamine , elution , high performance liquid chromatography , phase (matter) , organic chemistry , medicine
The concentrations of catecholamine‐related compounds in body fluids reflect sympathetic nerve functions. Measuring the enzyme activity of these metabolic pathways will improve diagnosis since a variety of symptoms are reported. An isocratic elution system with two column switching valves was developed using three types of semi‐micro columns for fast chromatographic analysis of catecholamine related compounds. Columns are a pentyl‐bonded phase, 50 × 2.1 mm i.d., a phenylhexyl‐bonded phase, 100 × 2.1 mm i.d. and an octadecyl‐bonded phase, 100 × 2.1 mm i.d. The separation of 20 standard compounds was achieved within 25 min using reversed‐phase ion‐pair liquid chromatography with an electrochemical detector. This new system was applied for analysis of catecholamine‐related compounds in pig brain, since pigs are a widely used animal model for transgenic manipulation of neural genes, and MHPG (or VMA), DOPAC, DOPA, NE, EP, DA, 5HTP and 5HIAA were quantified. Copyright © 2002 John Wiley & Sons, Ltd.