A sensitive capillary GC‐MS method for analysis of topiramate from plasma obtained from single‐dose studies
Author(s) -
Conway Jeannine M.,
Birnbaum Angela K.,
Marino Susan E.,
Cloyd James C.,
Remmel Rory P.
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1749
Subject(s) - topiramate , chemistry , chromatography , pharmacokinetics , extraction (chemistry) , pharmacology , epilepsy , medicine , psychiatry
ABSTRACT Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC‐MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid‐phase extraction and were quantified by GC‐MS analysis. The topiramate standard curves were split from 0.1 to 4 µg/mL and from 4 to 40 µg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies. Copyright © 2012 John Wiley & Sons, Ltd.