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Characterization of multiple absorbed constituents in rats after oral administration of Paederia scandens decoction
Author(s) -
Jiang Weixin,
Jin Di,
Li Zhixiong,
Sun Zhaolin,
Chen Mingcang,
Wu Bin,
Huang Chenggang
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1743
Subject(s) - decoction , chemistry , traditional medicine , oral administration , chromatography , pharmacology , medicine
Paederia scandens (Lour.) Merri. (Jishiteng in Chinese) is a Chinese traditional medicine widely used in treating various diseases. However, its active components have remained unknown. In the present study, a rapid and sensitive method by high‐performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC‐MS n ) techniques was employed to investigate the absorbed constituents in rats after oral administration of Paederia scandens decoction. By comparing their MS data with those of authentic compounds and published data, a total of six compounds (paederosid, 1; paederosidic acid, 2; paederosidic acid methyl ester, 3; 6‐hydroxy geniposide, 4; asperuloside, 5; and deacetyl asperuloside, 6) were identified in the P . scandens decoction samples. In addition, a total of seven compounds, including three iridoid glucosides and four of their metabolites, were identified in rat urine samples after administration. In addition, six compounds, including four iridoid glucosides and two of their metabolites, were identified in rat serum samples after administration. Our results significantly narrow the range of potentially active compounds in P . scandens decoction, and build a solid foundation for future research on its mechanism. Copyright © 2011 John Wiley & Sons, Ltd.