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Quantitative determination of lisinopril in human plasma by high performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study
Author(s) -
Qin Feng,
Wang Dan,
Yang Shuyan,
Jing Lijuan,
Xiong Zhili,
Li Famei
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1715
Subject(s) - chemistry , chromatography , protein precipitation , lisinopril , selected reaction monitoring , electrospray ionization , mass spectrometry , formic acid , tandem mass spectrometry , pharmacokinetics , triple quadrupole mass spectrometer , liquid chromatography–mass spectrometry , calibration curve , detection limit , high performance liquid chromatography , methanol , angiotensin converting enzyme , medicine , organic chemistry , radiology , blood pressure
A rapid, selective and sensitive high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) method was developed to determine lisinopril in human plasma. Sample pretreatment involved a one‐step protein precipitation with methanol of 0.1 mL plasma. Analysis was performed on an Inertsil ODS‐3 column (2.1 × 50 mm i.d., 3 µm) with mobile phase consisting of methanol–water (containing 0.2% formic acid; 55:45, v/v). The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode via an electrospray ionization source. Each plasma sample was chromatographed within 2.5 min. The linear calibration curves for lisinopril were obtained in the concentration range of 1.03–206 ng/mL ( r 2 ≥ 0.99) with a lower limit of quantification of 1.03 ng/mL. The intra‐ and inter‐day precisions (relative standard deviation) were not higher than 11%, and accuracy (relative error) was within ±6.8%, determined from quality control samples for lisinopril, which corresponded to the guidance of the Food and Drug Administration. The method described herein was fully validated and successfully applied to the pharmacokinetic study of lisinopril tablets in healthy male volunteers after oral administration. Copyright © 2011 John Wiley & Sons, Ltd.