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Validated high‐performance liquid chromatographic enantioseparation of selenomethionine using isothiocyanate based chiral derivatizing reagents
Author(s) -
Bhushan Ravi,
Dubey Rituraj
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1689
Subject(s) - chemistry , diastereomer , chromatography , derivatization , reagent , isothiocyanate , acetonitrile , high performance liquid chromatography , detection limit , selenium , organic chemistry
A high‐performance liquid chromatographic (HPLC) method for enantioseparation of selenomethionine (SeMet) was developed using two isothiocyanate‐based chiral derivatizing reagents [( R )‐methyl benzyl isothiocyanate (MBIC) and ( S )‐1‐(1‐naphthyl) ethyl isothiocyanate (NEIC)] and UV detection. Diastereomers of selenomethionine were synthesized either via stirring (using MBIC) or by microwave irradiation (using NEIC). Derivatization conditions were optimized and the synthesized diastereomers were successfully resolved using triethyl ammonium phosphate buffer and acetonitrile on a reversed‐phase column. The method was validated for accuracy, precision and limit of detection. The mechanism of separation is also discussed. Copyright © 2011 John Wiley & Sons, Ltd.