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Analysis of in vitro oxidized human LDL phospholipids by solid‐phase extraction and micellar electrokinetic capillary chromatography
Author(s) -
Yang YingLing,
Chong ChinPong,
Tsai MingHua,
Liu MineYine
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1684
Subject(s) - chemistry , chromatography , micellar electrokinetic chromatography , solid phase extraction , electrospray ionization , phospholipid , mass spectrometry , extraction (chemistry) , electrospray , elution , capillary electrophoresis , biochemistry , membrane
Phospholipids of in vitro oxidized human low‐density lipoproteins (LDL) were separated by two different solid‐phase extraction (SPE) methods. One of the two methods was designed to test the effects of gradient elution. This SPE method isolated more phospholipids from in vitro oxidized LDL than the other one according to the results of liquid chromatography and electrospray ionization mass spectrometry (LC ESI‐MS) analysis. A micellar electrokinetic capillary chromatography (MEKC) method was also used to analyze phospholipids separated by SPE. The results of MEKC and LC ESI‐MS were consistent for the major phospholipid classes, including PC, lysoPC, PE, PI and PS. The MEKC profiles showed significant differences for native and oxidized LDL phospholipids. Therefore, the unique combination of SPE and MEKC methods showed dramatic distinctions between native and in vitro oxidized human LDL phospholipids. The combination also shows great potential for rapid analysis of in vivo oxidized human LDL phospholipids in the future. Copyright © 2011 John Wiley & Sons, Ltd.