LC‐MS/MS method for the quantification of myo ‐ and chiro ‐inositol as the urinary biomarkers of insulin resistance in human urine

A simple LC‐MS/MS method has been developed and validated for the quantification of endogenous myo ‐ and chiro ‐inositol in human urine. myo ‐ and chiro ‐Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient ( n =  3) was greater than 0.9991 over the range 0.05–25.0 µg/mL with the weighted (1/ C 2 ) least square method. Precision (%RSD) and accuracy (%RE) were 0–10.0% and 0–6.0% for the intra‐day assay ( n =  5) and 0–14.3% and 0–10.0% for the inter‐day assay ( n =  5). myo ‐ and chiro ‐Inositol have been shown to be stable in human urine stored at room temperature and for three freeze–thaw cycles. Copyright © 2011 John Wiley & Sons, Ltd.