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LC‐MS/MS method for the quantification of myo ‐ and chiro ‐inositol as the urinary biomarkers of insulin resistance in human urine
Author(s) -
Kim ByoungHyoun,
Park JinYoung,
Jang Jae Bum,
Moon Dong Cheul
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1682
Subject(s) - urine , chemistry , chromatography , urinary system , inositol , coefficient of variation , endocrinology , biochemistry , receptor , medicine
A simple LC‐MS/MS method has been developed and validated for the quantification of endogenous myo ‐ and chiro ‐inositol in human urine. myo ‐ and chiro ‐Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient ( n = 3) was greater than 0.9991 over the range 0.05–25.0 µg/mL with the weighted (1/ C 2 ) least square method. Precision (%RSD) and accuracy (%RE) were 0–10.0% and 0–6.0% for the intra‐day assay ( n = 5) and 0–14.3% and 0–10.0% for the inter‐day assay ( n = 5). myo ‐ and chiro ‐Inositol have been shown to be stable in human urine stored at room temperature and for three freeze–thaw cycles. Copyright © 2011 John Wiley & Sons, Ltd.