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Salivary peptidome in type 1 diabetes mellitus
Author(s) -
Caseiro Armando,
Vitorino Rui,
Barros António S.,
Ferreira Rita,
CalheirosLobo Maria João,
Carvalho Davide,
Duarte José Alberto,
Amado Francisco
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1677
Subject(s) - saliva , zymography , proteases , western blot , chemistry , diabetes mellitus , matrix metalloproteinase , extracellular matrix , type 2 diabetes , type 2 diabetes mellitus , endocrinology , cathepsin , salivary proteins , medicine , biochemistry , enzyme , gene
Diabetic patients show a high susceptibility to oral diseases of inflammatory, catabolic and chronic nature with potential impact on saliva composition. In this study, our purpose was to characterize type 1 diabetes‐induced alterations in the salivary peptidome aiming to find prospective biomarkers for type 1 diabetes oral health evaluation. Peptidomic analysis of saliva from controls ( n = 5) and type 1 diabetic patients ( n = 5) were performed by liquid chromatography followed by mass spectrometry. The proteolytic activity and metalloproteinases expression was accessed by zymography and slot blot analysis, respectively. Data evidenced a significant increase in the percentage of peptides in diabetic patients paralleled by a higher proteolytic activity, compared with healthy individuals. The nonsalivary gland protein fragments identified in saliva were mainly derived from collagen and extracellular matrix proteins, namely collagen type I. The cleavage site frequency analysis showed significant differences between healthy and type 1 diabetic individuals, highlighting the activity of proteases such as matrix metalloproteinase‐9 and cathepsin D. Our results highlight salivary collagen fragments as potential biomarkers to follow up diabetes‐related oral damage. Copyright © 2011 John Wiley & Sons, Ltd.