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Highly sensitive LC‐MS/MS‐ESI method for simultaneous quantitation of albendazole and ricobendazole in rat plasma and its application to a rat pharmacokinetic study
Author(s) -
Sharma Kuldeep,
Kandaswamy Murugesh,
Mithra Chandan,
Meena Ashok Kumar,
Giri Sanjeev,
Rajagopal Sriram,
Mullangi Ramesh
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1654
Subject(s) - chromatography , chemistry , protein precipitation , ammonium acetate , pharmacokinetics , phenacetin , high performance liquid chromatography , pharmacology , medicine
A highly sensitive and specific LC‐MS/MS‐ESI method was developed for simultaneous quantification of albenadazole (ABZ) and ricobendazole (RBZ) in rat plasma (50 μL) using phenacetin as an internal standard (IS). Simple protein precipitation was used to extract ABZ and RBZ from rat plasma. The chromatographic resolution of ABZ, RBZ and IS was achieved with a mobile phase consisting of 5 m m ammonium acetate (pH 6) and acetonitrile (20:80, v/v) at a flow rate of 1 mL/min on a Chromolith RP‐18e column. The total chromatographic run time was 3.5 min and the elution of ABZ, RBZ and IS occurred at 1.66, 1.50 and 1.59 min, respectively. A linear response function was established for the ranges of concentrations 2.01–2007 and 6.02–6020 ng/mL for ABZ and RBZ, respectively. The intra‐ and inter‐day precision values for ABZ and RBZ met the acceptance as per FDA guidelines. ABZ and RBZ were stable in battery of stability studies, viz. bench‐top, auto‐sampler and freeze–thaw cycles. The developed assay was applied to a pharmacokinetic study in rats. Copyright © 2011 John Wiley & Sons, Ltd.