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In vivo metabolism study of polygalic acid in rat using HPLC‐ESI‐MS n
Author(s) -
Ling Yun,
Wu Bin,
Wang Ke,
Liu Huihui,
Chen Luying,
Fan Mingsong,
Sun Zhaolin,
Huang Chenggang
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1650
Subject(s) - chemistry , chromatography , formic acid , high performance liquid chromatography , electrospray ionization , solid phase extraction , metabolite , urine , tandem mass spectrometry , extraction (chemistry) , mass spectrometry , electrospray , acetonitrile , elution , biochemistry
A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi‐stage tandem mass spectrometry (HPLC‐ESI‐MS n ). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C 18 solid‐phase extraction cartridge, and then these pretreated samples were injected into a reversed‐phase C 18 column with a gradient elution program, whereas acetonitrile–0.5% aqueous formic acid was used as mobile phase and detected by an on‐line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (Δ M ), retention times and full‐scan MS n spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC‐ESI‐MS n ) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples. Copyright © 2011 John Wiley & Sons, Ltd.