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Determination of daumone in mouse plasma by HPLC/MS‐MS
Author(s) -
Noh Keumhan,
Park Jong Hee,
Kim Minkyu,
Jung Mankil,
Lee Hwa Jeong,
Kwon Kwangil,
Kang Wonku,
Ha Hunjoo
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1649
Subject(s) - chemistry , chromatography , protein precipitation , bioanalysis , electrospray ionization , high performance liquid chromatography , analyte , tandem mass spectrometry , mass spectrometry
Daumone, a pheromone secreted by Caenorhabditis elegans , is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS‐MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed‐phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5‐week repeated oral administration of daumone in mice. Copyright © 2011 John Wiley & Sons, Ltd.