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Determination of N ‐acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection
Author(s) -
Song Ziyu,
Ge Dan,
Ishii Kana,
Yamada Hiroshi,
Toriumi Kazuya,
Watanabe Hiroyuki,
Nabeshima Toshitaka,
Fukushima Takeshi
Publication year - 2012
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1639
Subject(s) - chemistry , olfactory bulb , high performance liquid chromatography , nucleus accumbens , chromatography , striatum , derivatization , fluorescence , hippocampus , prefrontal cortex , biochemistry , central nervous system , endocrinology , dopamine , neuroscience , medicine , physics , receptor , cognition , quantum mechanics , biology
The concentration of brain N ‐acetylaspartic acid (NAA) in mice was determined by high‐performance liquid chromatography (HPLC) using fluorescence detection after pre‐column derivatization with 4‐ N , N ‐dimethylaminosulfonyl‐7‐ N ‐(2‐aminoethyl)amino‐2,1,3‐benzoxadiazole (DBD‐ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2 ± 4.0, n = 8) and lowest in the hippocampus (42.8 ± 1.6, n = 8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area. Copyright © 2011 John Wiley & Sons, Ltd.