Determination of  N ‐acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection | Zendy

Song Ziyu | Zendy; Ge Dan | Zendy; Ishii Kana | Zendy; Yamada Hiroshi | Zendy; Toriumi Kazuya | Zendy; Watanabe Hiroyuki | Zendy; Nabeshima Toshitaka | Zendy; Fukushima Takeshi | Zendy

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Determination of N ‐acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Author(s) -

Song Ziyu,

Ge Dan,

Ishii Kana,

Yamada Hiroshi,

Toriumi Kazuya,

Watanabe Hiroyuki,

Nabeshima Toshitaka,

Fukushima Takeshi

Publication year - 2012

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1639

Subject(s) - chemistry , olfactory bulb , high performance liquid chromatography , nucleus accumbens , chromatography , striatum , derivatization , fluorescence , hippocampus , prefrontal cortex , biochemistry , central nervous system , endocrinology , dopamine , neuroscience , medicine , physics , receptor , cognition , quantum mechanics , biology

The concentration of brain N ‐acetylaspartic acid (NAA) in mice was determined by high‐performance liquid chromatography (HPLC) using fluorescence detection after pre‐column derivatization with 4‐ N , N ‐dimethylaminosulfonyl‐7‐ N ‐(2‐aminoethyl)amino‐2,1,3‐benzoxadiazole (DBD‐ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2 ± 4.0, n = 8) and lowest in the hippocampus (42.8 ± 1.6, n = 8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area. Copyright © 2011 John Wiley & Sons, Ltd.

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