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Quantification of lurasidone, an atypical antipsychotic drug, in rat plasma with high‐performance liquid chromatography with tandem mass spectrometry
Author(s) -
Koo TaeSung,
Kim SooJin,
Lee Jongjoo,
Ha DongJin,
Baek Myoungki,
Moon Hongsik
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1625
Subject(s) - chemistry , chromatography , formic acid , lurasidone , electrospray ionization , liquid chromatography–mass spectrometry , mass spectrometry , tandem mass spectrometry , detection limit , selected reaction monitoring , protein precipitation , acetonitrile , antipsychotic , programming language , schizophrenia (object oriented programming) , computer science
A liquid chromatography–tandem mass spectrometric (LC/MS/MS) method was developed for the determination of an atypical antipsychotic drug, lurasidone, in rat plasma. The method involves the addition of acetonitrile and ziprasidone (internal standard) solution to plasma samples, followed by centrifugation. An aliquot of the supernatant was diluted with water and directly injected into the LC/MS/MS system. The separations were performed on a column packed with octadecylsilica (5 μm, 2.0 × 50 mm) with 0.1% formic acid and 0.1% formic acid in acetonitrile as mobile phase and the detection was performed using tandem mass spectrometry by multiple‐reaction monitoring via an electrospray ionization source. The standard curve was linear ( r = 0.9982) over the concentration range 0.002–1 μg/mL. The intra‐ and inter‐assay precisions were 1.7 and 8.6%, respectively. The accuracy range was from 90.3 to 101.8%. The lower limit of quantification was 2.0 ng/mL using 50 μL of rat plasma sample. The developed analytical method was successfully applied to the pharmacokinetic study of lurasidone in rats. Copyright © 2011 John Wiley & Sons, Ltd.