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Imitation of biomembranes on the basis of cholic acid and endogenic thermostable protein complex in biopartitioning micellar chromatography
Author(s) -
Rukhadze Marina,
Dzidziguri Diana,
Giorgobiani Nana,
Kerkenjia Salome
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1610
Subject(s) - cholic acid , chemistry , chromatography , imitation , basis (linear algebra) , biochemistry , bile acid , psychology , social psychology , geometry , mathematics
The structure of biomembranes was imitated by introducing nonionic surfactant polyoxyethylene (23) dodecylether, cholic acid and endogenic thermostable protein complex (14–65 kDa) into the mobile phase. The influence of concentration of these additives on the retention of the model compounds was studied. The competing interaction of cholic acid and endogenic thermostable protein complex in the lipid bilayer model was revealed on the basis of chromatographic data. The values of efficiency of the chromatographic column regarding solutes were increased by addition of endogenic thermostable protein complex to the mobile phase containing Brij‐35 and cholic acid. Copyright © 2011 John Wiley & Sons, Ltd.