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Determination of bisphenol A in rat brain by microdialysis and column switching high‐performance liquid chromatography with fluorescence detection
Author(s) -
Sun Yen,
Nakashima Mihoko N.,
Takahashi Masakatsu,
Kuroda Naotaka,
Nakashima Kenichiro
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.161
Subject(s) - microdialysis , chemistry , chromatography , high performance liquid chromatography , acetonitrile , bisphenol a , derivatization , fluorescence , methanol , reagent , fluorescence spectroscopy , analytical chemistry (journal) , extracellular , biochemistry , physics , organic chemistry , quantum mechanics , epoxy
A sensitive column switching HPLC‐fluorescence detection for determination of bisphenol A (BPA) in rat brain by coupling with microdialysis was developed. A microdialysis probe was inserted into the hypothalamus of rat brain and an artificial cerebrospinal fluid was used for perfusion. BPA in brain dialysate was subjected to a fluorescent derivatization with 4‐(4,5‐diphenyl‐1 H ‐imidazol‐2‐yl)benzoyl chloride (DIB‐Cl), and the excess reagent was removed by a column‐switching technique. Separation was carried out on two ODS semimicro‐columns with the mobile phase of acetonitrile–H 2 O–methanol–tetrahydrofuran (55:10:35:2.5, v/v) and acetonitrile–0.1 M acetate buffer (pH 3.0)–methanol (35:10:55, v/v) at a flow rate of 0.10 and 0.15 mL/min for a precolumn and a separation column, respectively. Fluorescence intensity was monitored at 475 nm with excitation of 350 nm. BPA could be sensitively detected at 0.3 ppb in 60 µL brain microdialysate at a signal‐to‐noise ratio of 3. By the proposed method, concentrations of BPA in rat brain and plasma were monitored for 8 h after single i.v. or oral administration. It is proved that BPA is capable of penetrating the blood–brain barrier. The ratio of the area under the concentration–time curve of BPA in rat brain to that in blood was estimated to be about 3.0–3.8%. Copyright © 2002 John Wiley & Sons, Ltd.