Premium
Application of LC‐MS/MS method for the in vivo metabolite determination of oleuropein after intravenous administration to rat
Author(s) -
Zhou Ting,
Qian Tianxiu,
Wang Xiaoying,
Li Xianen,
Cao Li,
Gui Shuangying
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1609
Subject(s) - chemistry , chromatography , metabolite , formic acid , oleuropein , in vivo , urine , tandem mass spectrometry , electrospray , extraction (chemistry) , liquid chromatography–mass spectrometry , mass spectrometry , electrospray ionization , ethyl acetate , selected reaction monitoring , biochemistry , antioxidant , microbiology and biotechnology , biology
A highly sensitive, specific and simple LC‐MS/MS method was developed to investigate in vivo bio‐transformation of oleuropein in rat. Rat urine samples collected after the intravenous administrations were determined using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative‐ion mode. The assay procedure involves a simple liquid–liquid extraction of parent oleuropein and the metabolite from rat urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.80 mL/min on an RP‐C 18 column with a total run time of 30 min. This method has been successfully applied to simultaneous determination of oleuropein and its metabolite in rat urine. Oxygenation was found to be the major metabolic pathway of the oleuropein in rat after intravenous administration. Copyright © 2011 John Wiley & Sons, Ltd.