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Screening and analysis of aconitum alkaloids and their metabolites in rat urine after oral administration of aconite roots extract using LC‐TOFMS‐based metabolomics
Author(s) -
Tan Guangguo,
Lou Ziyang,
Jing Jing,
Li Wuhong,
Zhu Zhenyu,
Zhao Liang,
Zhang Guoqing,
Chai Yifeng
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1607
Subject(s) - aconitum , metabolomics , chemistry , traditional chinese medicine , traditional medicine , alkaloid , urine , aconitine , pharmacology , chromatography , ranunculaceae , biochemistry , medicine , biology , botany , stereochemistry , alternative medicine , pathology
Aconite roots are popularly used in herbal medicines in China. Many cases of accidental and intentional intoxication with this plant have been reported; some of these are fatal because the toxicity of aconitum is very high. It is thus important to detect and identify aconitum alkaloids in biofluids. In this work, an improved method employing LC‐TOFMS with multivariate data analysis was developed for screening and analysis of major aconitum alkaloids and their metabolites in rat urine following oral administration of aconite roots extract. Thirty‐four signals highlighted by multivariate statistical analyses including 24 parent components and 10 metabolites were screened out and further identified by adjustment of the fragmentor voltage to produce structure‐relevant fragment ions. It is helpful for studying aconite roots in toxicology, pharmacology and forensic medicine. This work also confirmed that the metabolomic approach provides effective tools for screening multiple absorbed and metabolic components of Chinese herbal medicines in vivo . Copyright © 2011 John Wiley & Sons, Ltd.