An improved LC‐MS/MS method for quantitative determination of metolazone in human plasma and its application to a pharmacokinetic study | Zendy

Jia Yanyan | Zendy; Zhang Yan | Zendy; Wang Chao | Zendy; Wang Zhirui | Zendy; Liu Yu | Zendy; Wang Jingwen | Zendy; Wen Aidong | Zendy

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An improved LC‐MS/MS method for quantitative determination of metolazone in human plasma and its application to a pharmacokinetic study

Author(s) -

Jia Yanyan,

Zhang Yan,

Wang Chao,

Wang Zhirui,

Liu Yu,

Wang Jingwen,

Wen Aidong

Publication year - 2011

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1583

Subject(s) - chemistry , chromatography , pharmacokinetics , distilled water , extraction (chemistry) , methanol , cmax , elution , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , pharmacology , medicine , organic chemistry

A simple, rapid and accurate liquid chromatography–tandem mass spectrometry method has been developed. After a liquid–liquid extraction procedure, samples were chromatographed on an Agilent TC‐C 18 (150 × 4.6 mm, 5 μm) column using an isocratic elution mobile phase composed of methanol and distilled water (70:30, v/v) at a flow rate of 0.5 mL/min. After single‐dose administration of 0.5, 1 and 2 mg metolazone, the t 1/2 values were 6.6 ± 2.8, 7.9 ± 1.2 and 7.6 ± 1.9 h, respectively. The pharmacokinetic parameters of multiple doses (1 mg metolazone) were as follows: t 1/2 was 8.9 ± 1.0 h; C max was 22.4 ± 5.0 ng/mL; and AUC 0–48 was 156.8 ± 31.6 ng h/mL. Copyright © 2011 John Wiley & Sons, Ltd.

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