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Capillary electrophoresis of Cr(VI) reducer Arthrobacter oxydans
Author(s) -
Tsibakhashvili Nelly Ya.,
Asatiani Nino V.,
Abuladze Marina K.,
Birkaya Barbara G.,
Sapojnikova Nelly A.,
Mosulishvili Liguri M.,
Holman HoiYing N.
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.157
Subject(s) - chemistry , capillary electrophoresis , electrophoresis , chromatography , buffer (optical fiber) , arthrobacter , capillary action , bacteria , phase (matter) , biochemistry , materials science , enzyme , telecommunications , organic chemistry , biology , computer science , composite material , genetics
Rapid and effective separation of bacteria Arthrobacter oxydans was performed using capillary electrophoresis. For optimal separation of bacteria the influence of buffer concentration, pH and applied voltage were studied. It was found that the most appropriate conditions for electrophoretic mobility measurements are as follows: applied voltage 6–14 kV; buffer concentration 5–10 m M pH 6–8. At the stationary phase of growth there are always two main heterogeneous peaks. They are connected with the morphology of bacteria as well as with cell aggregation. The heterogeneity of samples may be explained by surface modifications of bacterial cells. Copyright © 2002 John Wiley & Sons, Ltd.