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LC‐MS in analytical toxicology: some practical considerations
Author(s) -
Couchman Lewis,
Morgan Phillip E.
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1566
Subject(s) - chemistry , analyte , chromatography , sample preparation , mass spectrometry , liquid chromatography–mass spectrometry , complex matrix , matrix (chemical analysis) , analytical chemistry (journal)
Abstract Liquid chromatography, coupled with single‐stage or tandem mass spectrometry, is a powerful tool increasingly used in analytical toxicology. However, the atmospheric pressure ionization processes involved are complex, and subject to interference from matrix components, for example. Further, the techniques used in sample preparation, chromatography and mass analysis are developing rapidly. An understanding of the advantages and limitations of LC‐MS ensures appropriate analyses are performed, and that reliable results are generated. Consideration should be given to the influence of the sample preparation and chromatographic conditions on the ionization of the analyte at the mass spectrometer interface. This review aims to provide some practical guidance and examples to aid method development for commonly encountered analytes in analytical toxicology. Copyright © 2010 John Wiley & Sons, Ltd.