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RP‐HPLC‐DAD for simultaneous estimation of mahanine and mahanimbine in Murraya koenigii
Author(s) -
Pandit Subrata,
Kumar Mythies,
Ponnusankar S.,
Pal B. C.,
Mukherjee Pulok K.
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1561
Subject(s) - murraya , chemistry , chromatography , high performance liquid chromatography , rutaceae , traditional medicine , botany , biology , medicine
Murraya koenigii leaves (Rutaceae) are widely used as food condiments in various food preparations in India. They possess a wide range of biological activities including antioxidant, antibacterial, anticancer, hypoglycemic and hypolipidemic activity. A rapid reverse‐phase high‐performance liquid chromatography (RP‐HPLC) method has been developed for quantitative estimation of mahanine and mahanimbine, two major bioactive alkaloids in this plant. The amounts of mahanine and mahanimbine were detected as 9.56 ± 1.04 and 4.32 ± 0.81% w/w in the extract, with the retention times of 6.26 ± 0.66 and 10.40 ± 0.95 minutes. The limits of detection and quantification were estimated to be 29.30 and 81.12 µg/mL and 1.67 and 6.31 µg/mL, respectively. This specific and precise validated method can be useful for the routine analysis and quantitative determination of mahanine and mahanimbine in this therapeutically potent medicinal plant. Copyright © 2011 John Wiley & Sons, Ltd.