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Determination of tryptophan and kynurenine in human plasma by liquid chromatography–electrochemical detection with multi‐wall carbon nanotube‐modified glassy carbon electrode
Author(s) -
Liu Lihong,
Chen Ying,
Zhang Yulin,
Wang Fang,
Chen Zilin
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1550
Subject(s) - chemistry , chromatography , glassy carbon , high performance liquid chromatography , kynurenine , carbon nanotube , detection limit , elution , tryptophan , electrode , electrochemistry , analytical chemistry (journal) , cyclic voltammetry , nanotechnology , materials science , biochemistry , amino acid
A novel method was developed for the simultaneous determination of kynurenine and tryptophan by high‐performance liquid chromatography with electrochemical detection at multi‐wall carbon nanotube (MWCNT)‐modified glassy carbon electrode. The separation and detection conditions were optimized. The typical HPLC experiments were conducted by using a reversed‐phase ODS column with a mobile phase consisting of stock acetate buffer (pH 5)–methanol (4:1, v/v) using an isocratic elution at the flow rate of 1.0 mL/min. The obtained LODs for kynurenine and tryptophane were 0.5 and 0.4 µmol/L, respectively. The analytical method for human plasma samples was validated and confirmed by LC‐UV and LC‐MS. The recoveries were in the range of 84.8–110%, and the precision was lower than 5.9%. Copyright © 2010 John Wiley & Sons, Ltd.