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Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry
Author(s) -
Santa Tomofumi
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1548
Subject(s) - derivatization , chemistry , chromatography , electrospray ionization , mass spectrometry , reagent , electrospray , tandem mass spectrometry , liquid chromatography–mass spectrometry , direct electron ionization liquid chromatography–mass spectrometry interface , chemical ionization , ionization , organic chemistry , ion
Liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) is one of the most prominent analytical techniques owing to its inherent selectivity and sensitivity. In LC/ESI‐MS/MS, chemical derivatization is often used to enhance the detection sensitivity. Derivatization improves the chromatographic separation, and enhances the mass spectrometric ionization efficiency and MS/MS detectability. In this review, an overview of the derivatization reagents which have been applied to LC/ESI‐MS/MS is presented, focusing on the applications to low molecular weight compounds. Copyright © 2010 John Wiley & Sons, Ltd.