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A highly sensitive LC‐MS/MS method for the determination of S ‐raclopride in rat plasma: application to a pharmacokinetic study in rats
Author(s) -
Punde Suresh P. S, Ravindra Ramachandra,
Gupta Manish,
Dixit Abhishek,
Giri Sanjeev,
Rajagopal Sriram,
Mullangi Ramesh
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1547
Subject(s) - chemistry , chromatography , formic acid , phenacetin , electrospray ionization , pharmacokinetics , extraction (chemistry) , selected reaction monitoring , mass spectrometry , electrospray , tandem mass spectrometry , pharmacology , medicine
A highly sensitive and rapid assay method has been developed and validated for the estimation of S ‐(−)‐raclopride ( S ‐RCP) in rat plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive ion mode. The assay procedure involves a simple liquid–liquid extraction technique for extraction of S ‐RCP and phenacetin (internal standard, IS) from rat plasma. Chromatographic separation was achieved with 0.2% formic acid : acetonitrile (80:20, v/v) at a flow rate of 0.30 mL/min on a Phenomenex Prodigy C 18 column with a total run time of 4.5 min. The MS/MS ion transitions monitored were 347.2 → 112.1 for S ‐RCP and 180.1 → 110.1 for IS. Method validation and pre‐clinical sample analysis were performed as per FDA guidelines and the results met the acceptance criteria. The lower limit of quantitation achieved was 0.05 ng/mL and the linearity range was extended from 0.05 to 152 ng/mL in rat plasma. The intra‐day and inter‐day precisions were 0.23–10.5 and 3.74–7.29%, respectively. This novel method was applied to a pharmacokinetic study of S ‐RCP in rats. Copyright © 2010 John Wiley & Sons, Ltd.