Premium
Towards clinical proteomics analysis
Author(s) -
Imai Kazuhiro,
Koshiyama Akiyo,
Nakata Katsunori
Publication year - 2010
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1541
Subject(s) - chemistry , chromatography , derivatization , proteomics , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , reproducibility , bottom up proteomics , quantitative proteomics , protein mass spectrometry , biochemistry , gene
To understand accurate protein dynamics, a highly reproducible proteomics analytical method is required. The acquired thus knowledge will lead to the diagnosis, treatment and protection against diseases. This review deals with proteomics analysis from a view of sample pre‐treatment, sensitivity and reproducibility of the methods, including two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE), liquid chromatography–tandem mass spectrometry (LC‐MS/MS). and fluorogenic derivatization (FD)‐LC‐MS/MS. Copyright © 2010 John Wiley & Sons, Ltd.