Towards clinical proteomics analysis | Zendy

Imai Kazuhiro | Zendy; Koshiyama Akiyo | Zendy; Nakata Katsunori | Zendy

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Towards clinical proteomics analysis

Author(s) -

Imai Kazuhiro,

Koshiyama Akiyo,

Nakata Katsunori

Publication year - 2010

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1541

Subject(s) - chemistry , chromatography , derivatization , proteomics , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , reproducibility , bottom up proteomics , quantitative proteomics , protein mass spectrometry , biochemistry , gene

To understand accurate protein dynamics, a highly reproducible proteomics analytical method is required. The acquired thus knowledge will lead to the diagnosis, treatment and protection against diseases. This review deals with proteomics analysis from a view of sample pre‐treatment, sensitivity and reproducibility of the methods, including two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE), liquid chromatography–tandem mass spectrometry (LC‐MS/MS). and fluorogenic derivatization (FD)‐LC‐MS/MS. Copyright © 2010 John Wiley & Sons, Ltd.

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