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A rapid and simple assay for lamotrigine in serum/plasma by HPLC, and comparison with an immunoassay
Author(s) -
Morgan Phillip E.,
Fisher Danielle S.,
Evers Richard,
Flanagan Robert J.
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1515
Subject(s) - chemistry , chromatography , lamotrigine , immunoassay , high performance liquid chromatography , quantitative analysis (chemistry) , antibody , biology , neuroscience , immunology , epilepsy
Monitoring serum/plasma concentrations of lamotrigine may be useful under certain circumstances. An HPLC column packed with strong cation‐exchange (SCX)‐modified microparticulate silica together with a 100% methanol eluent containing an ionic modifier permits direct injection of sample extracts. An HPLC‐UV method developed using this principle for the measurement of serum/plasma lamotrigine is simple, sensitive and selective. The analysis time is less than 5 min. Intra‐ and inter‐assay precision and accuracy meet acceptance criteria, and sample stability, and potential interferences from other compounds have been evaluated. There was good agreement with consensus mean results from external quality assessment samples ( n = 32). Analysis of patient samples ( n = 115) using the HPLC method and the Seradyn QMS® Lamotrigine immunoassay showed that the immunoassay over‐estimated lamotrigine by 21% on average. Copyright © 2010 John Wiley & Sons, Ltd.