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Metabonomic study on women of reproductive age treated with nutritional intervention: screening potential biomarkers related to neural tube defects occurrence
Author(s) -
Jiang Zhiting,
Liang Qionglin,
Wang Yong,
Zheng Xiaoying,
Pei Lijun,
Zhang Ting,
Wang Yiming,
Luo Guoan
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1512
Subject(s) - chemistry , docosahexaenoic acid , carnitine , neural tube , multivitamin , micronutrient , vitamin , metabolome , vitamin c , food science , physiology , chromatography , biochemistry , metabolomics , medicine , polyunsaturated fatty acid , fatty acid , biology , embryo , organic chemistry , microbiology and biotechnology
Nutritional intervention is effective in reducing the risk of neural tube defects (NTDs). To determine the effects of nutritional supplementation on human metabolism, a metabonomic study was carried out on 96 women of reproductive age. Subjects with nutritional intervention were given fortified wheat flour (containing folic acid, vitamin B 1 , vitamin B 2 , ferric sodium edetate and zinc oxide) for 8 months. Serum metabolic fingerprinting was detected via ultraperformance liquid chromatography in tandem with time‐of‐flight mass spectrometry (UPLC‐TOF MS), and data acquired was processed by multivariate statistical analysis. The result revealed a significant difference between the control and intervention group. Twenty potential biomarkers, including fructose 6‐phosphate, sphingosine 1‐phosphate, docosahexaenoic acid and hexadecanoic acid, were located and identified by the accurate mass measurement of TOF MS. These compounds are believed to be functionally related to anti‐oxidative competence in vivo . In conclusion, metabonomics study is a valuable approach in exploring the effect mechanism of nutritional intervention on NTD prevention. Copyright © 2010 John Wiley & Sons, Ltd.