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A sensitive fluorescence reagent, 2‐[2‐(7H‐dibenzo[a,g]carbazol‐7‐yl)‐ethoxy]ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high‐performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source
Author(s) -
Li Guoliang,
Sun Zhiwei,
Song Cuihua,
Xia Lian,
Zheng Jie,
Suo Yourui,
You Jinmao
Publication year - 2011
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1504
Subject(s) - chemistry , ethyl chloroformate , chromatography , reagent , derivatization , detection limit , high performance liquid chromatography , chloroformate , fluorescence , organic chemistry , physics , quantum mechanics
Recent researches shows that amino acids (AA) are not only cell signaling molecules but are also regulators of gene expression and the protein phosphorylation cascade. More precise analysis of AA composition is reckoned to be one of the most important applications in the biomedical and pharmaceutical fields. In this paper, we develop a sample, sensitive and mild method using 2‐[2‐(7H‐dibenzo[a,g]carbazol‐7‐yl)‐ethoxy]ethyl chloroformate (DBCEC) as A labeling reagent for AA determination by high‐performance liquid chromatography (HPLC) with fluorescence detection (FLD) and identification with mass spectroscopy. The maximum excitation and emission wavelengths for DBCEC‐AA derivatives were 300 and 395 nm, respectively. This method, in conjunction with a gradient elution, offered a baseline resolution of 20 AA on a reversed‐phase Hypersil BDS C 18 column. LC separation for the derivatized AA showed good reproducibility, and all AA were found to give excellent linear responses with correlation coefficients > 0.9993. The calculated detection limits with a 25.0 fmol injection of each AA (at a signal‐to‐noise ratio of 3:1) ranged from 2.62 to 22.6 fmol. This method was applied to determine the AA composition in Saussurea involucrate and Artemisia capillaris Thunb. Meanwhile, this method exhibits a powerful potential for trace analysis of AA from biomedicine, foodstuff and other complex samples. Copyright © 2010 John Wiley & Sons, Ltd.