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Analysis of glycoproteins and the oligosaccharides thereof by high‐performance capillary electrophoresis—significance in regulatory studies on biopharmaceutical products
Author(s) -
Kakehi Kazuaki,
Kinoshita Mitsuhiro,
Nakano Miyako
Publication year - 2002
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.150
Subject(s) - capillary electrophoresis , glycoprotein , chemistry , glycan , chromatography , oligosaccharide , biopharmaceutical , mass spectrometry , electrophoresis , gel electrophoresis , biochemistry , microbiology and biotechnology , biology
This review describes the recent development in the analysis of glycoproteins using capillary electrophoresis with various separation techniques, and focuses especially on the analysis of recombinant glycoprotein pharmaceuticals. We include the analysis of glycoprotein multiforms (ie glycoform) as well as glycan analysis. The relationship between glycoprotein multiforms and oligosaccharide distributions in a glycoprotein sample is also discussed. Further, recent development in capillary electrophoresis–mass spectrometry is described. Copyright © 2002 John Wiley & Sons, Ltd.